乙酰胆碱对三种人垂体腺瘤细胞增殖及凋亡的影响

迟素敏1,*, 李承新2, 刘亚莉1, 朱运龙1, 顾建文3, 杜亮1, 王复周1

第四军医大学 1生理学教研室、2西京医院全军皮肤性病中心、3西京医院脑外科, 西安  710032

摘要:  为了解乙酰胆碱(acetylcholine, ACh)在人垂体腺瘤发生、发展中的作用, 本研究首先观察了人垂体腺瘤细胞内是否存在合成ACh必需的胆碱乙酰转移酶, 并应用MTT实验、［3H］TdR掺入实验、细胞周期分析和TUNEL法测定了ACh对体外培养的人垂体无功能瘤、催乳素瘤和生长激素瘤等三种瘤细胞增殖和凋亡的影响。结果发现: (1)三种垂体腺瘤细胞中均有胆碱酯酶的表达, 但明显少于正常垂体; (2)ACh对三种类型的人垂体腺瘤细胞增殖代谢的影响相类似, 不同浓度的ACh能明显抑制体外培养的三种人垂体腺瘤细胞的增殖, 呈明显的剂量效应关系, 同时ACh能减少垂体腺瘤细胞进入S、G2期的细胞比例, 而使处于G1期的细胞比例增加; (3)ACh的这种作用可被阿托品阻断, 但不受筒箭毒的影响; (4)ACh对体外培养的三种人垂体腺瘤细胞凋亡无明显影响。该结果提示, ACh可能以旁分泌或自分泌的方式作用于垂体前叶细胞, 对垂体腺瘤细胞增殖分化有调控作用, 而且是通过ACh M受体来实现的。

关键词:  乙酰胆碱; 垂体; 腺瘤; 增殖; 凋亡

学科分类号:  Q45; R335

The effect of acetylcholine on the proliferation and apoptosis of three kinds of cultured human pituitary adenoma cells

CHI Su-Min1,*, LI Cheng-Xin2, LIU Ya-Li1, ZHU Yun-Long1, GU Jian-Wen3, DU Liang1, WANG Fu-Zhou1

1Department of Physiology, the Fourth Military Medical University, Xi′an  710032; 2Center of Dermatology of Chinese PLA and 3Department of Neurosurgery, Xijing Hospital, the Fourth Military Medical University, Xi′an  710032

Abstract:  In order to elucidate the effect of acetylcholine (ACh) on the occurrence and development of human pituitary adenoma, it was firstly observed whether there exists choline acetyl transferase (ChAT) which is necessary for the synthesis of acetylcholine in the cells of human pituitary adenoma, and then MTT method, ［3H］TdR incorporation, cell cycle analysis and TUNEL were employed to estimate the influence of ACh on the proli

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