表1 原癌基因c-erbB-1和c-erbB-2在正常脑组织,胶质瘤及胶质瘤细胞系中的表达
tab 1 Expression of proto-oncogenes c-erbB-1 and c-erbB-2 in normal brain tissues, gliomas, and glioma cell lines
Type nc-erbB-1
c-erbB-2
- + ++ +++ PI/% - + ++ +++ PI/% NB 8 8 0 0 0 0.0 8 0 0 0 0.0G
Grade Ⅰ 12 6 6 0 0 50.0 3 6 3 0 75.0 Grade Ⅱ 16 5 5 5 1 68.8 2 6 7 1 87.5 Grade Ⅲ 11 2 1 5 3 81.8 1 2 4 4 90.9 Grade Ⅳ 13 1 1 4 7 92.3 1 1 3 8 92.3 Low grade 28 11 11 5 1 60.7 5 12 10 1 82.1 High grade 24 3 2 9 10 87.5 2 3 7 12 91.7 Total 52 14 13 14 11 73.1 7 15 17 13 86.5 GCL 2 0 0 1 1 100.0 0 0 2 0 100.0NB：normal brain tissue, G：glioma, GCL：glioma cell line, PI：positive rate
2.3 c-erbB-1和c-erbB-2免疫组化结果综合分析 二者均表达者35例,均阴性者4例,c-erbB-1表达而c-erbB-2阴性者3例,c-erbB-2表达而c-erbB-1阴性者10例,二者表达水平密切相关（P＜0.05).
图1 原纤维型星形细胞瘤(Ⅱ级)c-erbB-1蛋白染色
fig 1 c-erbB-1 protein staining in fibrillary astrocytoma (grade Ⅱ)
DAB as chromogen, hematoxylin counterstain, the positive cells show brown-yellow staining in their cell cytoplasms and membranes, SABC method ×400
图2 多形性胶质母细胞瘤(Ⅳ级)c-erbB-1蛋白染色
fig 2 c-erbB-1 protein staining in glioblastoma multiforme (grade Ⅳ)
DAB as chromogen, hematoxylin counterstain, the positive cells show brown-yellow staining in their cell cytoplasms and membranes, SABC method ×400
图3 原浆型星形细胞瘤(Ⅱ级)c-erbB-2蛋白染色
fig 3 c-erbB-2 protein staining in protoplasmic astrocytoma (grade Ⅱ)
DAB as chromogen, hematoxylin counterstain, the positive cells show brown-yellow staining in their cell cytoplasms and membranes, SABC method ×400
图4 多形性胶质母细胞瘤(Ⅳ级)c-erbB-2蛋白染色
fig 4 c-erbB-2 protein staining in glioblastoma multiforme (grade Ⅳ)
DAB as chromogen, hematoxylin counterstain, the positive cells show brown-yellow staining in their cell cytoplasms and membranes, SABC method ×400